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All times are CEST (UTC + 2)
| Astana – Chelsea | 0:0 (16:30) | | | København – Hearts | 0:0 (18:45) | | | Fiorentina – Linec | 0:0 (18:45) |
| Başakşehir – Heidenheim | 0:0 (18:45) | | | Legia – Lugano | 0:0 (18:45) | | | Helsinki – Molde | 0:0 (18:45) |
| Petrocub Hîncești – Betis | 0:0 (18:45) | | | Dinamo Minsk – Larne | 0:0 (18:45) | | | Noah – APOEL | 0:0 (18:45) |
| Gent – Bačka Topola | 0:0 (21:00) | | | Omonia Nikósie – Rapid | 0:0 (21:00) | | | Shamrock – Banja Luka | 0:0 (21:00) |
| New Saints – Pan. Ateny | 0:0 (21:00) | | | Mladá Boleslav – Jagiellonia | 0:0 (21:00) | | | Pafos – Celje | 0:0 (21:00) |
| Víkingur Reykjavík – Djurgården | 0:0 (21:00) |
| Roma – Braga | 0:0 (18:45) | | | Hoffenheim – FCSB | 0:0 (18:45) | | | Ludogorec – Alkmaar | 0:0 (18:45) |
| Malmö – Galatasaray AŞ | 0:0 (18:45) | | | Olympiacos – Twente | 0:0 (18:45) | | | PAOK – Ferencváros | 0:0 (18:45) |
| Plzeň – Man Utd. | 0:0 (18:45) | | | Maccabi Tel Aviv – RFS Riga | 0:0 (21:00) | | | Rangers – Tottenham | 0:0 (21:00) |
| Real Sociedad – Dynamo Kyiv | 0:0 (21:00) | | | Slavia – Anderlecht | 0:0 (21:00) | | | Ajax – Lazio | 0:0 (21:00) |
| Bodø/Glimt – Beşiktaş | 0:0 (21:00) | | | Elfsborg – Qarabağ FK | 0:0 (21:00) | | | Porto – Midtjylland | 0:0 (21:00) |
| Lyon – Frankfurt | 0:0 (21:00) |
before game
Ajax Amsterdam 
0:0
(-:-)
S. S. Lazio |
Welcome. Game starts at 21:00.
(15:30:17)
|
Goals:
Referees:
Attendance:
Penalty missed:
Ajax Amsterdam:
Subs:
Coach:
S. S. Lazio:
Subs:
Coach:
Subs:
Coach:
S. S. Lazio:
Subs:
Coach:
Referees:
Attendance: